Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Long non-coding RNA LINC00346 promotes pancreatic cancer growth and gemcitabine resistance by sponging miR-188-3p to derepress BRD4 expression

Fig. 1

Overexpression of LINC00346 promotes the growth of pancreatic cancer. a Measurement of LINC00346 expression levels in pancreatic cancer cells. *P < 0.05 vs. HPDE6c7 pancreatic epithelial cells. b Real-time PCR analysis confirmed the upregulation of LINC00346 in PANC-1 and Capan-1 cells transfected with vector or LINC00346-expressing plasmid. c MTT assay was performed to assess the proliferation of pancreatic cancer cells transfected with indicated constructs. d EdU incorporation assay demonstrated that LINC00346 overexpression increased the fraction of EdU-positive proliferating cells (green). Nuclei stained with DAPI (blue). Scale bar = 100 μm. e Pancreatic cancer cells transfected with indicated constructs were subjected to colony formation assay. f Xenograft tumor studies. PANC-1 cells transfected with vector or LINC00346-expressing plasmid were subcutaneously injected into nude mice, and tumor growth curves were plotted (n = 4). *P < 0.05 vs. the vector group

Back to article page