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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Long non-coding RNA LINC00346 promotes pancreatic cancer growth and gemcitabine resistance by sponging miR-188-3p to derepress BRD4 expression

Fig. 2

Knockdown of LINC00346 exerts growth-suppressive effects on pancreatic cancer cells. a Real-time PCR analysis validated the knockdown of LINC00346 in PANC-1 and Capan-1 cells transfected with control shRNA (shCtrl) or LINC00346-targeting shRNA. b Assessment of cell proliferation by EdU incorporation assay. Scale bar = 100 μm. c Colony formation assay was used to determine the colony-forming ability. *P < 0.05 vs. the shCtrl group. d Cell cycle distribution by flow cytometry after PI staining. Numbers indicate mean ± s.d. percentage of cells in each phase of the cell cycle. e Western blot analysis of indicated proteins

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