Fig. 3

Depletion of LINC00346 enhances gemcitabine sensitivity in pancreatic cancer cells. a The IC50 value for gemcitabine was significantly lower in LINC00346-depleted cells than that in control cells. b Flow cytometric analysis of apoptosis in PANC-1 and Capan-1 cells after indicated treatments. Upper, representative dot plots of Annexin-V/PI staining for apoptosis in PANC-1 cells. Here, 40 μM gemcitabine was used. c Measurement of caspase-3 activity in the cells treated as in (b). d LINC00346-depleted and control PANC-1 cells were subcutaneously injected into nude mice and then treated with gemcitabine, and tumor growth curves were plotted (n = 4). Inserts are representative tumors from 4 mice. e Tumor weight was determined for each group. ^*P < 0.05