Fig. 5From: Long non-coding RNA LINC00346 promotes pancreatic cancer growth and gemcitabine resistance by sponging miR-188-3p to derepress BRD4 expressionLINC00346 rescues the inhibitory effect of miR-188-3p on pancreatic cancer cells. a Real-time PCR analysis confirmed the upregulation of miR-188-3p in PANC-1 and Capan-1 cells transfected with vector or miR-188-3p-expressing plasmid. b Cell proliferation determined by EdU incorporation assay. c Colony formation assay in cells transfected with indicated constructs. d miR-188-3p overexpression significantly enhanced gemcitabine cytotoxicity, which was abolished by overexpression of LINC00346. e Ago2-based RIP assay performed in PANC-1 cells co-transfected with LINC00346 and miR-188-3p. *P < 0.05. f Analysis of the intracellular distribution of LINC00346 and miR-188-3p in PANC-1 cells by FISH. Scale bar = 20 μm. Nuclei were counterstained with DAPI (blue)Back to article page