Fig. 6

LINC00346 counteracts miR-188-3p to promote BRD4 expression. a Prediction of a target site for miR-188-3p in the 3’-UTR of BRD4 mRNA based on the online program (http://www.targetscan.org/vert_71/). b Luciferase reporter assay confirmed that the reporter containing the 3’-UTR of BRD4 was repressed by overexpression of miR-188-3p. ^*P < 0.05; n.s. indicates no significance. c Western blot analysis of BRD4 protein in PANC-1 cells transfected with indicated constructs. Numbers indicate fold change in BRD4 protein levels relative to control cells. d Real-time PCR analysis of miR-188-3p levels in PANC-1 and Capan-1 cells transfected with control shRNA (shCtrl) or LINC00346-targeting shRNA (shLINC00346). ^*P < 0.05. e Western blot analysis of BRD4 protein in PANC-1 and Capan-1 cells transfected with indicated constructs