Fig. 3

Knockdown of GK5 inhibits PC9R cell proliferation, increases apoptosis, and induces cell cycle arrest. a PC9R cells expressing GK5-shRNA (shGK5–1 or − 2) or negative control shRNA (shNEG) were treated with 1 μM gefitinib and then stained with EdU and Hoechst 33342. b The percentage of EdU-positive cells was higher in the control group than in the GK5-silenced groups. c Flow cytometry on PC9R cells expressing shGK5–1, − 2 or shNEG. Cells treated with 1 μM gefitinib and stained with APC-annexin V/DAPI. d GK5 silencing increased apoptosis in PC9R cells. e Cell cycle analyzed by flow cytometry. f Flow cytometry on cell cycle of PC9R cells expressing shGK5–1, − 2 or shNEG. Cells were treated with 1 μM gefitinib, and the number of cells in different phases of cell cycle was detected. The data are representative of three experiments. * p < 0.05 vs. shNEG