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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: FBXO22 promotes the development of hepatocellular carcinoma by regulating the ubiquitination and degradation of p21

Fig. 2

FBXO22 promotes proliferation and tumorigenesis of HCC cells in vitro and in vivo. (a) shFBXO22 effectively decreased FBXO22 expression in HLF and HepG2 cells. Transfection with scrambled shRNA (shvec) was used as negative control, and GAPDH was used as a loading control. Knockdown of FBXO22 expression effectively inhibited cell growth (b), foci formation (c), and tumor formation in nude mice (g, h), while FBXO22 was stably overexpressed in Hep3B and LM3 cells as detected by western blot analysis. Transfection with empty vectors (vector) were used as negative control, and GAPDH was used as a loading control (d). Overexpression of FBXO22 promoted cell growth (e) and foci formation (f). The results are expressed as the means ± standard error of the mean (SEM) of three independent experiments. Xenograft Tumor weight and tumor volume are shown as means ± SEM (g). The results are expressed as the means ± standard error of the mean (SEM) of three independent experiments. Orthotopic tumor volume are shown as means ± SEM (h). Representative IHC images of FBXO22, p21 and ki-67 expression in xenograft tumors (400×, magnification) (I)

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