Fig. 3

FBXO22 regulates the protein levels of p21. (a) HLF and HepG2 cells were transfected with the indicated constructs, total protein was extracted and subjected to western blotting using anti-FBXO22, anti-p21, and anti-GAPDH antibodies, respectively. (b) Quantification of the p21 levels relative to GAPDH expression (c) HLF and HepG2 cells were transfected with the indicated constructs, total RNA was extracted and subjected to real-time qPCR using primers specific for FBXO22, p21, or GAPDH. (d) Hep3B and LM3 cells were transfected with the indicated constructs, total protein was extracted and subjected to western blotting using anti-FBXO22, anti-p21, and anti-GAPDH antibodies, respectively. (e) 293 T cells were transfected with Flag-FBXO22 at different dosages. Total protein was extracted and subjected to western blotting using anti-Flag, anti-p21, and anti-GAPDH antibodies, respectively. (f, g) FBXO22-depleted cell lines were transfected with the indicated constructs, total protein was extracted and subjected to western blotting using anti-FBXO22, anti-p21, and anti-GAPDH antibodies, respectively