Fig. 4

FBXO22 interacts with p21. (a) HEK293T cells transfected with vectors expressing Myc-FBXO22 and Flag-p21 were treated with Mg132 (5 μg/ml) for 6 h. The cell lysates were subjected to immunoprecipitation with anti-Flag or anti-Myc antibodies. (b) HEK293T cells transfected with vectors expressing Myc-FXBO22 and Flag-p53 were treated with Mg132 (5 μg/ml) for 6 h. The cell lysates were subjected to immunoprecipitation with anti-Flag or anti-Myc antibodies. (c) HLF cells were treated with Mg132 (5 μg/ml) for 6 h. The cell lysates were subjected to immunoprecipitation with anti-FBXO22 or anti-p21 antibodies. (d) HepG2 cells were treated with Mg132 (5 μg/ml) for 6 h. The cell lysates were subjected to immunoprecipitation with anti-FBXO22 or anti-p21 antibodies