Fig. 1

miR-205 enhances PCa cell sensitivity to radiation. (a) (Left panel) qRT-PCR showing miR-205 expression kinetics normalized to RNU-48 in DU145 and PC-3 cells up to 240 h upon miR-205 reconstitution, compared to control cells. Data are reported as relative quantity (RQ) ± SD with respect to Neg cells. All RQ values are statistically significant considering p < 0.0001. (Right panel) Clonogenic cell survival of DU145 and PC-3 cells transiently transfected with miR-205 or Neg and exposed to increasing doses of irradiation (2, 4, 6 and 8 Gy). The surviving fraction is reported as mean ± SD values from 3 independent experiments. (b) (Left panel) qRT-PCR showing miR-205 amount normalized to RNU-48 in Vec miR-205-transfected DU145 cells, compared to control cells. Data are reported as relative quantity (RQ) ± SD with respect to Neg cells. (Right panel) Clonogenic cell survival of DU145 cells stably transfected with miR-205 or Neg vector and exposed to irradiation (2, 4, 6 and 8 Gy). The surviving fraction is reported as mean ± SD values from 3 independent experiments. The level of significance was represented as *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test