Fig. 4

PKCε and ZEB1 down-regulation phenocopies miR-205-induced radiosensitization. (a) (Left panel) qRT-PCR showing PKCε mRNA amount in miR-205- or siPKCε-transfected DU145 cells, compared to control cells, normalized to GAPDH. Data are reported as relative quantity (RQ) ± SD with respect to Neg cells. (Middle panel) Western blot analysis showing PKCε protein amount in DU145 cells upon transfection with Neg, miR-205 or siPKCε. β-Actin was used as equal protein loading controls. Cropped images of selected proteins are shown. (Right panel) Clonogenic cell survival of DU145 cells transfected with miR-205 or siPKCε. The surviving fractions following the indicated doses of irradiation are reported as mean ± SD values from 3 independent experiments. (b) (Left panel) qRT-PCR showing ZEB1 mRNA amount in miR-205- or siZEB-transfected DU145 cells, compared to control cells, normalized to GAPDH. Data are reported as relative quantity (RQ) ± SD with respect to Neg cells. (Middle panel) Western blot analysis, and corresponding relative quantification, showing ZEB1 protein amount in DU145 cells upon transfection with Neg, miR-205 or siZEB1. β-Actin was used as equal protein loading controls. Cropped images of selected proteins are shown. (Right panel) Clonogenic cell survival of DU145 cells transfected with miR-205 or siZEB1. The surviving fractions are reported as mean ± SD values from 3 independent experiments. The level of significance was represented as *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test