Fig. 2

TRPM7 silencing inhibit the migration, invasion and wound healing of ovarian cancer cells and the metastasis of ovarian cancer in mice. SKOV3 and OVCAR3 cells were transfected with plasmid for scrambled RNA or TRPM7-specific shRNA expression to establish SKOV3-sh, SKOV3-TRPM7-sh, OVCAR3-sh and OVCAR3-TRPM7-sh cells. (a) Western blot and qRT-PCR analyses of TRPM7 expression. (b-c) The EGF-induced migration, invasion and wound healing of SKOV3-sh, SKOV3-TRPM7-sh, OVCAR3-sh and OVCAR3-TRPM7-sh cells were determined by transwell migration and invasion and wound healing assays. (d) TRPM7 silencing decreases the growth of ovarian cancer and promotes the survival of mice bearing ovarian cancer. BALB/c nude mice were randomized and injected intravenously with SKOV3-sh or SKOV3-TRPM7-sh cells. At 30th post inoculation, the lung tissues were dissected from each group (n = 5) of mice and imaged. The remaining mice were monitored for their death (n = 5 per group). (e) The lung metastatic ovarian tumors were histologically examined and the expression of TRPM7 in the tumor tissues was determined by immunohistochemistry. Data are representative images or expressed as the mean ± SD of each group from at least three separate experiments