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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: TRPM7 promotes the epithelial–mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling

Fig. 4

TRPM7 silencing reduces the intracellular calcium concentrations in ovarian cancer cells. (a)The fluorescence curves of the SKOV3 and OVCAR3 cells. ‘a’, cells that did not receive any treatment, and were exposed to Ca2 + −free HANK’s solution after uploaded the Fluo-8 AM fluorescence kit. The fluorescence curve of those cells is used as baseline for [Ca2+]I; ‘b’, the cells were treated with MK886 for 24 h, and exposed to HBSS (with CaCl2) after using the Fluo-3 AM fluorescence kit; ‘c’, the cells did not receive any treatment, but were exposed to HBSS (with CaCl2) after using the Fluo-8 AM fluorescence kit; ‘d’, the cells did not receive any treatment and after using the Fluo-8 AM fluorescence kit, the fluorescence curve of those cells was used as a negative control. Following exposure to HBSS (with CaCl2), the fluorescence curve of [Ca2+]I of the cells that TRPM7 silencing was higher than baseline, but lower than the cells that did not receive treatment. This indicated that TRPM7 silencing led to a significant decrease in the [Ca2+]I of the SKOV3 and OVCAR3 cells. (b) Fluorescent imaging the [Ca2+]i. SKOV3 and OVCAR3 cells were labeled with Fluo-8 AM and examined under a fluorescent microscope. Data are representative images or expressed as the mean ± SD of each group from three separate experiments

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