Fig. 7

TRPM7 silencing inhibits the EMT by attenuating the calcium -mediated PI3K / AKT signaling in ovarian cancer cells. (a) Inhibition of TRPM7 expression alters the morphology of ovarian cancer cells. SKOV3-sh, SKOV3-TRPM7-sh, OVCAR3-sh and OVCAR3-TRPM7-sh cells were treated with vehicle or BAPTA-AM for 24 h. Similarly, SKOV3 and OVCAR3 cells were treated with vehicle or MK886 and/or BAPTA-AM for 24 h. The cell morphology was observed under a light microscope. (b) Inhibition of TRPM7 expression modulates the PI3K/AKT inhibitor or IGF-induced morphology in ovarian cancer cells. SKOV3 and OVCAR3 cells were treated with vehicle, BAPTA-AM, and/or LY294002 or IGF for 24 h and the cell morphology was observed. (c, d) Western blot analysis of the EMT-related molecule expression in individual groups of cells. (e) Immunohistochemistry analysis of PI3K expression and AKT phosphorylation in ovarian cancer tissues. Data are representative images of each group of cells from three separate experiments