Fig. 3From: SNHG1 promotes malignant biological behaviors of glioma cells via microRNA-154-5p/miR-376b-3p- FOXP2- KDM5B participating positive feedback loopFOXP2 acted as an oncogene in both glioma tissues and cells, miR-154-5p and miR-376b-3p inhibited malignant progression of glioma cells by binding to the FOXP2–3′-UTR. a FOXP2 protein expression in glioma tissues of different grades and normal brain tissues (NBTs) with GAPDH as an endogenous control. Data were presented as the mean ± SD (n = 10, each group). **P < 0.01 vs. NBTs group. ##P < 0.01 vs. low grade group. b Western blot analysis of FOXP2 expression in U87 and U251 cells, with GAPDH as an endogenous control. **P < 0.01 vs. HA group. c Representative IHC assay patterns of FOXP2 expression in glioma tissues and normal brain tissues on tissue microarray sections. The photographs were taken at 400× magnification. d CCK-8 assay was performed to explore the proliferation effect of FOXP2 on U87 and U251 cells. e The apoptosis percentages of U87 and U251 cells were detected after FOXP2 overexpression or inhibition. f Effects of FOXP2 expression changes on migration and invasion of U87 and U251 cells. Scale bars represented 20 μm. For B, D, E and F, data were presented as the mean ± SD (n = 5, each group). **P < 0.01 vs. ex-NC group, ##P < 0.01 vs. sh-NC group. qRT-PCR and Western blot analysis revealed the negative correlation between miR-154-5p (g, h) or miR-376b-3p (j, k) and FOXP2 expression in glioma cells. Data were presented as the mean ± SD (n = 5, each group). **P < 0.01 vs. pre-NC group, ##P < 0.01 vs. anti-NC group. For luciferase reporter assay, the predicted miR-154-5p (i) or miR-376b-3p (l) binding sites in the 3′-UTR region of FOXP2 (FOXP2–3′-UTR-Wt) and the designed mutant sequence (FOXP2–3′UTR-Mut) were indicated. Renilla/firefly luciferase ratios were calculated and further normalized. **P < 0.01 vs. pre-NC groupBack to article page