Fig. 3
PRMT1 and PRMT5 regulates CFLARL independently of their enzymatic activity. a H1299 cells were transfected with the pEBG-CFLARL plasmids and co-transfected with pcDNA3.1 or pcDNA3.1-MYC-PRMT5. The cells were harvested and prepared for the GST pull-down assay after 24 h, and the arginine monomethylation of the CFLARL protein was detected by western blotting. b The symmetric arginine dimethylation of the CFLARL protein was detected by western blotting. c Cells were transfected with the pEBG-CFLARL plasmids and co-transfected with pcDNA3.1 or pcDNA3.1-MYC-PRMT1. The cells were harvested and prepared for the GST pull-down assay after 24 h, and the arginine monomethylation of the CFLARL protein was detected by western blotting. d The asymmetric arginine dimethylation of the CFLARL protein was detected by western blotting. e and f A549 cells were seeded in 6-well plates. PRMT5-WT and PRMT5-T139/144A plasmids or PRMT1-WT and PRMT1-G98R plasmids were transfected for 24 h, while pcDNA3.1 was transfected as control. Cells were harvested for western blot analysis