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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: The arginine methyltransferase PRMT5 and PRMT1 distinctly regulate the degradation of anti-apoptotic protein CFLARL in human lung cancer cells

Fig. 5

PRMT5 and PRMT1 regulate the turnover of CFLARL. a H460 cells were transfected with the pcDNA3.1 and pcDNA3.1-PRMT5 plasmids, and after 24 h, the cells were seeded again into a 6-well cell culture plate to ensure that each well contained identical cell densities. The cells were treated with 10 μg/ml CHX for 0 h, 1 h, 2 h, and 4 h. Then, the cells were harvested, and whole-cell protein lysates were prepared to detect the expression of CFLARL. b H460 cells were transfected with the pcDNA3.1 and pcDNA3.1-PRMT1 plasmids. Regarding the subsequent experiments, refer to (A). ACTB or GAPDH expression was detected as a loading control. The dynamics of CFLARL turnover were calculated using software Image J, and the curves were drawn with excel

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