Fig. 1

Knockdown of FUS suppressed GEC angiogenesis in U87 glioma. (a-b) The mRNA and protein expression levels of FUS in ECs and GECs were evaluated by qRT-PCR and western blot. GAPDH was used as an endogenous control. IDVs represents integrated density values. Data are presented as the means ± SD (n = 5, each group). ^**P < 0.01 vs. ECs group. (c) The effects of FUS knockdown on GEC viability were detected by the CCK-8 assay. Data are presented as the means ± SD (n = 5, each group). ^**P < 0.01 vs. FUS (−) NC group. (d) The effects of FUS knockdown on GECs were detected by the transwell assay. Data are presented as the means ± SD (n = 5, each group). ^**P < 0.01 vs. FUS (−) NC group. The scale bar represents 100 μm. (e) The effects of FUS knockdown on GECs tube formation were measured by the Matrigel tube formation assay (black arrow, tube structures; grey arrow, tube branches). Data are presented as the means ± SD (n = 5, each group). ^**P < 0.01 vs. FUS (−) NC group. The scale bar represents 100 μm. (f) The relative enrichment of circ_002136 in anti-IgG and anti-FUS was detected by an RNA-IP assay. Data are presented as the means ± SD (n = 3, each group). ^**P < 0.01 vs. anti-IgG group. (g) FUS and GAPDH protein levels immunoprecipitation with circ_002136 were evaluated by western blot. The expression levels of FUS and GAPDH proteins are shown. (h) The effects of FUS knockdown on circ_002136 expression levels were detected by qRT-PCR. Data are presented as the means ± SD (n = 5, each group). ^**P < 0.01 vs. FUS (−) NC group. (i) The effects of FUS knockdown on CDK11A expression levels were detected by qRT-PCR. Data are presented as the means ± SD (n = 5, each group)