Fig. 2

Knockdown of circ_002136 inhibited GEC angiogenesis in U87 glioma. (a) The relative expression of circ_002136 was detected in ECs and GECs by qRT-PCR. Data are presented as the means ± SD (n = 5, each group). ^**P < 0.01 vs. ECs group. (b) The relative expression of CDK11A was detected in ECs and GECs by qRT-PCR. Data are presented as the means ± SD (n = 5, each group). (c) The effects of circ_002136 knockdown on the viability of GECs were detected by the CCK-8 assay. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. circ_002136(−)NC group. (d) The effects of circ_002136 knockdown on the migration of GECs were determined by the transwell assay. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. circ_002136(−)NC group. The scale bar represents 100 μm. (E) The effects of circ_002136 knockdown on the tube formation of GECs were evaluated by the Matrigel tube formation assay. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. circ_002136(−)NC group. The scale represents 100 μm