Fig. 3

Over-expression of miR-138-5p suppressed GEC angiogenesis in U87 glioma. (a) The relative expression of miR-138-5p in ECs and GECs was detected by qRT-PCR. U6 was used as an internal control. Data are presented as the means ± SD (n = 5, each group). ^**P < 0.01 vs. ECs group. (b) The effects of miR-138-5p on the viability of GECs were determined by the CCK-8 assay. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. miR-138-5p(+)NC group; ^##P < 0.01 vs. miR-138-5p(−)NC group. (c) The effects of miR-138-5p on the migration of GECs were determined by the transwell assay. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. miR-138-5p(+)NC group; ^##P < 0.01 vs. miR-138-5p(−)NC group. The scale bar represents 100 μm. (d) The effects of miR-138-5p on tube formation of GECs were evaluated by the Matrigel tube formation assay. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. miR-138-5p(+)NC group; ^##P < 0.01 vs. miR-138-5p(−)NC group. The scale bar represents 100 μm