Fig. 2
From: SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production
SHMT1 expression is decreased in HCC and predicts poor prognosis of HCC patients. (a) qRT-PCR was performed to evaluate SHMT1 mRNA level in 120 pairs of HCC tissues and adjacent non-tumor tissues. (b) IHC staining was performed to evaluate SHMT1 protein level in 40 pairs of HCC tissues and adjacent non-tumor tissues. (c) qRT-PCR was performed to measure SHMT1 mRNA level in HCC cell lines and human immortalized hepatocyte LO2 cells. (d) Western blot was performed to measure SHMT1 mRNA level in HCC cell lines and human immortalized hepatocyte LO2 cells. (e) Kaplan-Meier analysis was performed for our HCC patients’ cohort to evaluate the association between SHMT1 level and patients’ overall survival. (f) Kaplan-meier analysis was performed for HCC patients’ cohort in TCGA database to evaluate the association between SHMT1 level and patients’ overall survival. *P < 0.05