Fig. 3
From: SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production
SHMT1 inhibits the migration, invasion, EMT and MMP2 production of HCC cells. Retrovirus encoding empty vector or SHMT1 vector were transduced into HCCLM3 cells. (a) qRT-PCR and (b) western blot were employed to evaluate the efficacy of retrovirus transduction. (c) Boyden chamber and transwell assay were employed to investigate the effect of SHMT1 overexpression on cell migration and invasion. Lentivirus encoding negative control shRNA or SHMT1 shRNA was transduced into Hep3B cells. (d) qRT-PCR and (e) western blot were employed to evaluate the efficacy of lentivirus transduction. (f) Boyden chamber and transwell assay were employed to investigate the effect of SHMT1 knockdown on cell migration and invasion. (g) qRT-PCR and (h) western blot were employed to evaluate the effect of SHMT1 overexpression on the expression level of E-cadherin, N-cadherin, Vimentin and MMP2. (i) qRT-PCR and (j) western blot were employed to evaluate the effect of SHMT1 knockdown on the expression level of E-cadherin, N-cadherin, Vimentin and MMP2. *P < 0.05