Fig. 6
From: SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production
SHMT1 suppresses NOX1 expression in HCC cells. (a) and (b) qRT-PCR was performed to examine the effect of SHMT1 overexpression and knockdown on the mRNA levels of NOX family. (c) Western blot was performed to examine the effect of SHMT1 overexpression and knockdown on the protein level of NOX1 in HCCLM3 and Hep3B cells. (e) IHC staining was performed to investigate the correlation between the protein levels of SHMT1 and NOX1 in HCC tissues. (f) Positive rates of NOX1 staining was compared between HCC tissues with positive SHMT1 staining and those with negative SHMT1 staining using Chi-square analysis. (g) Correlation between the IHC scores of SHMT1 protein and NOX1 protein was evaluated using Spearman rank analysis. *P < 0.05