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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production

Fig. 7

NOX1 promotes the migration, invasion, EMT and MMP2 production of HCC cells. Empty vector or NOX1 vector was transfected into Hep3B cells. (a) qRT-PCR and (b) western blot were used to evaluate the efficacy of NOX1 vector transfection, and to evaluate the effect of NOX1 overexpression on the expression level of E-cadherin, N-cadherin, Vimentin and MMP2. (c) DCFH-DA fluorescence assay was performed to investigate the effect of NOX1 overexpression on ROS production. (d) Boyden chamber and transwell assay were performed to investigate the effect of NOX1 overexpression on cell migration and invasion. Negative control siRNA or NOX1 siRNA was transfected into HCCLM3 cells. (d) qRT-PCR and (e) western blot were performed to evaluate the efficacy of NOX1 siRNA transfection, and to evaluate the effect of NOX1 knockdown on the expression level of E-cadherin, N-cadherin, Vimentin and MMP2. (f) DCFH-DA fluorescence assay was employed to investigate the effect of NOX1 knockdown on ROS production. (g) Boyden chamber and Transwell assay were performed to investigate the effect of NOX1 knockdown on cell migration and invasion. *P < 0.05

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