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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production

Fig. 8

NOX1 knockdown reverses the functional influence of SHMT1 knockdown on Hep3B cells. NOX1 siRNA or negative control siRNA was transfected into Hep3B cells with or without SHMT1 knockdown. (a) DCFH-DA fluorescence was performed to investigate whether NOX1 knockdown blocked the promoting effects of SHMT1 knockdown on ROS production of Hep3B cells. (b) Boyden chamber and transwell assay were performed to evaluate whether NOX1 knockdown blocked the promoting effects of SHMT1 knockdown on the migration and invasion of Hep3B cells. (c) and (d) qRT-PCR and Western blot were performed to examine whether NOX1 knockdown blocked the promoting effects of SHMT1 knockdown on EMT and MMP2 expression of Hep3B cells. *P < 0.05

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