Fig. 9
From: SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production
NOX1 overexpression abrogated the functional influence of SHMT1 overexpression on HCCLM3 cells. NOX1 vector or empty vector was transfected into HCCLM3 cells overexpressing SHMT1 or those in control group. (a) DCFH-DA fluorescence was performed to investigate whether NOX1 overexpression abrogated the decrease of ROS production induced by SHMT1 overexpression in HCCLM3 cells. (b) Boyden chamber and Transwell assay were performed to evaluate whether restoring NOX1 expression abrogated the decrease of cellular migration and invasion induced by SHMT1 overexpression in HCCLM3 cells. (c) and (d) qRT-PCR and Western blot were performed to examine whether restoring NOX1 expression abrogated the inhibitory effect of SHMT1 overexpression on EMT and MMP2 expression of HCCLM3 cells. *P < 0.05