Fig. 2

DRAM2 sub-location and expression in non-small cell lung cancer (NSCLC) cell lines. a. Immunofluorescence assays were performed to detect DRAM2 sub-locations in NSCLC cell lines. DRAM2 was located in the cytoplasm surrounding around nucleus. b. Relative expression of DRAM2 mRNA in six lung cancer cell lines and a normal bronchial cell line (HBE).Compared the expression of DRAM2 in those cell lines with HBE,A549 got no statistical significance,H1299 p = 0.0093,SK p = 0.049,H460 p = 0.0072,H661 p = 0.009,H292 p = 0.0055.*p < 0.05, **p < 0.01, ***p < 0.001. c. DRAM2 protein levels in NSCLC cell lines assessed by immunoblotting and analyzed with ImageLab software.The relative expression was quantified below