Fig. 2

TRIM14 increases migration/invasion in GBM cells by EMT. a Western blot analysis of TRIM14, N-cadherin, E-cadherin and Vimentin in LN229 and U251 cells transduced with shTRIM14–1, shTRIM14–2 or control shRNA. Equal loading is confirmed by β-actin levels. b Representative images of transwell invasion assay using LN229 and U251 cells transfected with the indicated shRNA. Quantification of transwell invasion assay is shown. ***p < 0.001; n = 3 experiment. c-d Representative images of wound healing assay using LN229 and U251 cells with the indicated shRNA. Quantification of wound healing assay is shown. ***p < 0.001, n = 3 experiments. e-f Representative images of 3D spheroid BME cell invasion assays using LN229 and U251 cells with the indicated shRNA. Quantification of 3D spheroid BME cell invasion assays is shown. ***p < 0.001, n = 3 experiments