Fig. 3

TRIM14 overexpression promotes migration/invasion in glioma cells by EMT. a Western blot analysis of TRIM14, N-cadherin, E-cadherin and Vimentin in HS683 and U87 cells transduced with vector or TRIM14. Equal loading is confirmed by β-actin levels. b Representative images of transwell invasion assay using HS683 and U87 cells transfected with vector or TRIM14. Quantification of transwell invasion assay is shown. ***p < 0.001; n = 3 experiment. c-d Representative images of wound healing assay using HS683 and U87 cells with vector or TRIM14. Quantification of wound healing assay is shown. ***p < 0.001, n = 3 experiments. e-f Representative images of 3D spheroid BME cell invasion assays using HS683 and U87 cells with vector or TRIM14. Quantification of 3D spheroid BME cell invasion assays is shown. ***p < 0.001, n = 3 experiments