Fig. 3
miR-767-5p is a target of circ_0000190 and promotes the progression of MM. a qRT-PCR analysis of miR-767-5p in human MM and normal healthy tissues. Tumor vs. Normal, P < 0.001. b qRT-PCR analysis ofmiR-767-5p in human MM and normal healthy peripheral blood. Tumor vs. Normal, P < 0.001. c Relativity analysis showed a negative correlation between circ_0000190 and miR-767-5p in human MM tissues. d Relativity analysis showed a negative correlation between circ_0000190 and miR-767-5p in human MM plasma. e qRT-PCR detects the effect of circ_0000190 and sh-circ_0000190 on the expression of miR-767-5p in MM. 1S and H929 cells. *** circRNA or sh-circRNA vs. control, P < 0.001. f The predicted binding sites for miR-767-5p in the 3’UTR of circ_0000190 and the mutations in the binding sites are shown. g Luciferase reporter assay of circ_00001903’UTR-wild type and mutant with miR-767-5p. ** miR-767-5p mimics vs. NC, P < 0.01. h The CCK-8 assay detects the effect of miR-767-5p mimics and inhibitor on cell viability of H929 cells. ** miR-767-5p mimics or inhibitor vs. control, P < 0.01. i The CCK-8 assay detects the effect of miR-767-5p mimics and inhibitor on cell viability of MM. 1S cells. ** miR-767-5p mimics or inhibitor vs. control, P < 0.01. j EdU labeling assay detects the effect of miR-767-5p mimics and inhibitor on cell proliferation. ** miR-767-5p mimics or inhibitor vs. control, P < 0.01. k Flow cytometry detects the effect of miR-767-5p mimics and inhibitor on cell cycle. ** miR-767-5p mimics or inhibitor vs. control, P < 0.01. l IF staining detects the effect of miR-767-5p mimics and inhibitor on the expression of MAPK4. m Western blot analysis detects the effect of miR-767-5p mimics and inhibitor on the expression of MAPK4, CDK6, CDK4, Cyclin D1 and Cyclin E, p21Cip1. **P < 0.01, ***P < 0.001