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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: CircRNA circ_0000190 inhibits the progression of multiple myeloma through modulating miR-767-5p/MAPK4 pathway

Fig. 5

MAPK4 is a target of miR-767-5p and inhibits progression of MM. a The three predicted binding sites for miR-767-5p in the 3’UTR of MAPK4 and the mutations in the binding sites are shown. b Luciferase reporter assay of MAPK43’UTR-wildtype and mutant with miR-767-5p. **, *** miR-767-5p mimics vs. NC, P < 0.01, P < 0.001. c Transfection efficiency of MAPK4 over-expression and knocking down were determined by qRT-PCR. *** MAPK4 or sh-MAPK4 vs. control, P < 0.001. d The CCK-8 assay detects the effect of MAPK4 over-expression and knocking down on cell viability of H929 cells. ** MAPK4 or sh-MAPK4 vs. control, P < 0.01. e The CCK-8 assay detects the effect of MAPK4 over-expression and knocking down on cell viability of MM. 1S cells. *, ** MAPK4 or sh-MAPK4 vs. control, P < 0.05, P < 0.01. f EdU labeling assay detects the effect of MAPK4 over-expression and knocking down on cell proliferation. **, *** MAPK4 or sh-MAPK4 vs. control, P < 0.01, P < 0.001. g Flow cytometry detects the effect of MAPK4 over-expression and knocking down on cell cycle of H929 cells. ** MAPK4 or sh-MAPK4 vs. control, P < 0.01. h Flow cytometry detects the effect of MAPK4 over-expression and knocking down on cell cycle of MM. 1S cells. ** MAPK4 or sh-MAPK4 vs. control, P < 0.01. i IF staining detects the effect of MAPK4 over-expression and knocking down on the expression of MAPK4. j Western blot analysis detects the effect of MAPK4 over-expression and knocking down on the expression of MAPK4, CDK6, CDK4, Cyclin D1 and Cyclin E, p21Cip1

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