Fig. 6
MiR-767-5p promotes progression of MM by inhibiting MAPK4. a The CCK8 assay detects the effect of MAPK4 and sh-MAPK4, miR-767-5p mimics and inhibitor on cell viability of H929 and MM. 1S cells. b EdU labeling assay detects the effect of MAPK4 and sh-MAPK4, miR-767-5p mimics and inhibitor on cell proliferation. c Flow cytometry detects the effect of MAPK4 and sh-MAPK4, miR-767-5p mimics and inhibitor on cell cycle. d IF staining detects the effect of MAPK4 and sh-MAPK4, miR-767-5p mimics and inhibitor on the expression of MAPK4. e Western blot analysis detects the effect of MAPK4 and sh-MAPK4, miR-767-5p mimics and inhibitor on the expression of MAPK4, CDK6, CDK4, Cyclin D1 and Cyclin E, p21Cip1.Different letters in the figure indicate statistically significant differences (P < 0.05)