Fig. 2
From: Bazedoxifene as a novel GP130 inhibitor for Colon Cancer therapy
Bazedoxifene inhibits colony formation and cell migration in human colon cancer cells. a: Colony formation assay was conducted in DLD-1, HCT-15, and HCT-116 cells as described in materials and methods. DLD-1, HCT-15, and HCT-116 cells were treated with bazedoxifene at indicated concentrations for 24 h, re-seeded at 1000 cells per well and cultured for 2–3 weeks to grow clones. b: A representative picture shows DLD-1 cells in a wound-healing assay. It was conducted by scratching the cells with a yellow pipette tip when DLD-1 cells grew into a monolayer. Then, cells were treated with bazedoxifene (15, 20 and/or 25 μM) and allowed to migrate into the scratched area for 24 h. Red arrows indicate a gap in the scratched area. c: The percentage of migrating area in wound-healing assay was quantified in DLD-1, HCT-15, and HCT-116 cells. (*, p < 0.05; **, p < 0.01; ***, p < 0.001)