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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma

Fig. 2

Endogenous expression of SNHG20 and effect of SNHG20 on the biological behaviors of glioma cells. a Expression levels of SNHG20 in NBTs, LGGTs and HGGTs. Data are presented as mean ± SD (NBTs (n = 15), LGGTs (n = 15), HGGTs (n = 15)). **P < 0.01 vs. NBTs group, ##P < 0.01 vs. LGGTs group. b Expression of SNHG20 in NHA, U87 and U251 cells. Data are presented as mean ± SD (n = 3, each group). **P < 0.01 vs. NHA group. c Expression levels of SNHG20 regulated by ZRANB2 in U87 and U251 cells. Data are presented as mean ± SD (n = 3, each group). *P < 0.05 vs. ZRANB2(−)-NC group, #P < 0.05 vs. ZRANB2(+)-NC group. d Protein levels of MMP1, MMP9 and VE-Cadherin regulated by SNHG20 in U87 and U251 cells. Representative protein expressions and corresponding IDVs of MMP1, MMP9, VE-Cadherin in U87 and U251 are shown; data are presented as mean ± SD (n = 3, each group). *P < 0.05 vs. SNHG20(−)-NC group, **P < 0.01 vs. SNHG20(−)-NC group, #P < 0.05 vs. SNHG20(+)-NC group, ##P < 0.01 vs. SNHG20(+)-NC group. e CCK-8 assay was used to measure the effect of proliferation on U87 and U251 cells by SNHG20. Data are presented as mean ± SD (n = 3, each group). *P < 0.05 vs. SNHG20(−)-NC group, **P < 0.01 vs. SNHG20(−)-NC group, #P < 0.05 vs. SNHG20(+)-NC group, ##P < 0.01 vs. SNHG20(+)-NC group. f Transwell assay was used to measure the effect of migration and invasion on U87 and U251 cells by SNHG20. Representative images and corresponding statistical plots are shown. Data are presented as mean ± SD (n = 3, each group). *P < 0.05 vs. SNGH20(−)-NC group, **P < 0.01 vs. SNHG20(−)-NC group, #P < 0.05 vs. SNHG20(+)-NC group, ##P < 0.01 vs. SNHG20(+)-NC group. Scale bars indicate 50 μm. g Three-dimensional culture was used to measure the effect of VM on U87 and U251 cells by SNHG20. Representative images and corresponding statistical plots are shown. Data are presented as mean ± SD (n = 3, each group). *P < 0.05 vs. SNHG20(−)-NC group, #P < 0.05 vs. SNHG20(+)-NC group. Scale bars indicate 50 μm

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