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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer

Fig. 5

miR-7 inhibited GC distant metastasis and improved overall survival in vivo. HGC-27 and MKN-28 cells were stalely transfected with indicated Lentivirus (MiR-7 and Control). After sorting purification, cells (2 × 106 /mice in 100 μl PBS buffer) were injected tail intravenously into the nu/nu mice (n = 8 mice/group). a and b Restoration of miR-7 in GC cells inhibited body weight loss in experimental metastasis mice. Post-transfection, body weight and the status of nude mice were monitored every 3 days and presented as mean ± SD (a). Representative images of the mice sizes are shown (b). c-f miR-7 markedly suppressed the lung and liver metastasis of GC cells. After indicated time point, lungs and livers from experimental mice were collected and metastatic tumor of the lung and liver surfaces were counted under a dissecting microscope (c and d) and were confirmed by H&E staining on sections from embedded lung and liver tissues (E and F). metastatic tumor was indicated by black arrow. Representative images of metastatic lung and liver tissues and H&E staining are shown. Scale bars indicate 50 μm and 200 μm. g miR-7 markedly improved overall survival (OS) in experimental metastasis mice. Kaplan-Meier analysis was used to analyze OS in established experimental metastasis mice (n = 10mice/group). Log-rank test was used for Kaplan-Meier survival analyses. Data are presented as mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001 between the indicated two groups determined by paired student’s t test

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