Fig. 7

miR-7 alleviates inflammation cells infiltration in GC metastasis. Experimental metastasis mice were established as described in Materials & Methods. Inflammation cells was evaluated in embedded metastatic lung and liver tissues by IHC staining using specific antibodies including CD45 (a), MPO (b), CD11c (c), F4/80 (d), CD11b (e) and GR-1 (f). Positive staining cells were counted from 5 high magnification fields (× 400) in metastatic tissues. Relative expression was normalized to 1 compared with control group. Representative IHC images are shown. *p < 0.05, **p < 0.01 and ***p < 0.001 between the indicated two groups determined by paired student’s t test. Scale bars: (main) 50 μm; (inset) 200 μm