Fig. 2

EVI1 influenced the cell proliferation and invasive capacity of NPC cells. (a) The EVI1 downregulation and overexpression efficiency was examined by a western blot assay. (b) Downregulation of EVI1 significantly decreased the cell proliferation ability, whereas overexpression of EVI1 increased the cell proliferation ability. (c) EVI1 downregulation decreased the NPC cell colony formation ability, whereas overexpression of EVI1 increased the cell colony formation ability. (d) Downregulation of EVI1 inhibited the invasive ability of 5-8F and CNE-2 cells, while overexpression of EVI1 markedly increased the invasive capacity of 6-10B cells. (e) Compared with sh-ctrl cell-derived xenograft tumors, sh-EVI1 cell-derived xenograft tumors grew more slowly. (f) The mean weight of sh-EVI1 cell-derived xenograft tumors was also significantly lower than that of sh-ctrl cell-derived xenograft tumors. (g) A Ki-67 staining assay revealed that EVI1 downregulation decreased the cell-derived xenograft proliferation rate. (h) sh-EVI1 cells formed fewer and smaller nodes per lung than sh-ctrl cells