Fig. 5
EVI1 regulated the Wnt/β-catenin signaling pathway in NPC. (a) EVI1 downregulation inhibited β-catenin expression in the total cell lysate and the nuclear fraction. In contrast, forced expression of EVI1 enhanced β-catenin expression in the total cell lysate and the nuclear fraction. (b) The Wnt/β-catenin activity was repressed when EVI1 was knocked down as revealed by a TOP/FOP Flash reporter assay (left panel). EVI1 elevated Wnt/β-catenin activity when EVI1 was overexpressed (right panel). (c) The expression levels of β-catenin downstream targets AXIN2 and c-myc were downregulated in sh-EVI1 cells and were upregulated in LV-EVI1 cells. (d) The β-catenin promoter region contains one putative EVI1-binding site. (e) A ChIP assay demonstrated that EVI1 bound to the β-catenin promoter. (f) EVI1 positively regulated the β-catenin promoter activity