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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: MiR-9 promotes tumorigenesis and angiogenesis and is activated by MYC and OCT4 in human glioma

Fig. 1

MiR-9 is frequently up-regulated in glioma tissues and facilitates glioma cell proliferation, migration and invasion. a MiR-9 expression levels were analyzed in the data from GSE4290 (N, n = 23; G, n = 153; left) and GSE15824 (N, n = 5; G, n = 30; right), respectively. N, normal tissues; G, glioma tissues. Data are represented as the mean ± s.d. (**P < 0.01 and ***P < 0.001). b MiR-9 expressions in the 3 normal (N) and 18 glioma (G) tissues were detected by qRT-PCR. Data are shown as the mean ± s.d. (*P < 0.05). c Proliferation of the A172 miR-9 mimic/NC cells was measured by MTT assay. Error bars represent the s.d. (*P < 0.05; n = 6 independent experiments). d Flow cytometry analysis was used to evaluate cell cycle of A172 miR-9 mimic/NC cells. Data are shown as the mean ± s.d. (*P < 0.05; n = 3 independent experiments). e Wound-healing assay was used to determine the migration of A172 miR-9 mimic/NC cells (upper). Photos were taken at 0, 24 and 48 h, respectively. Histogram was used for the statistical analysis of wound-healing assay for A172 miR-9 mimic/NC cells (lower). Scale bars represent 200 μm. Data are represented as the mean ± s.d. (*P < 0.05 and **P < 0.01; n = 3 independent experiments). f Migration (upper) and invasion (lower) of the A172 miR-9 mimic/NC cells were measured by the non-coated transwell and Matrigel-coated transwell assays, respectively. Scale bars represent 100 μm. Data are shown as the mean ± s.d. (*P < 0.05 and **P < 0.01; n = 3 independent experiments)

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