Fig. 4

MiR-9 induces glioma tumorigenesis and angiogenesis in vitro and in vivo. a Expression level of miR-9 in GL261 LV-miR-9 and GL261 LV-NC cells was detected by qRT-PCR. Data are shown as the mean ± s.d. (***P < 0.001; n = 3 independent experiments). b Proliferation ability of GL261 LV-miR-9 and GL261 LV-NC cells was measured via the colony formation assay. Data are presented as the mean ± s.d. (*P < 0.05; n = 3 independent experiments). c Brain sections of the mice from intracranial glioma model were used to perform the H&E staining and representative graphs were shown. Scale bars represent 1 mm. d Quantification of the tumor size. The tumor size was calculated according to the formula: (W² x L) / 2, W < L. Data are shown as the mean ± s.d. (*P < 0.05; n = 5 independent experiments). e Body weights of mice in the intracranial glioma model were measured in the 0, 3, 6, 9, 12 days after injection. Data are represented as the mean ± s.d. (*P < 0.05; n = 5 independent experiments). f Overall survival of the experimental mice was analyzed by the Kaplan-Meier survival curve. Statistical significance was measured through P log-rank test. g Brain sections of the mice from the intracranial glioma model were used to perform the CD31 IHC staining and representative graphs were shown. Scale bars represent 1 mm (left) and 50 μm (right)