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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MiR-9 promotes tumorigenesis and angiogenesis and is activated by MYC and OCT4 in human glioma

Fig. 5

COL18A1, THBS2, PTCH1 and PHD3 are the direct downstream biotargets of miR-9. a Diagram of the interaction between the miR-9 (seed site) and target mRNAs (3’-UTRs). The wild-type and mutant sequences of 3’-UTRs are listed. b Endogenous levels of COL18A1, THBS2, PTCH1 and PHD3 in glioma cell lines (A172, U87 and U251) were detected by qRT-PCR, respectively. Data are shown as the mean ± s.d.. c Dual-luciferase reporter assays were used to measure the relative luciferase activity of the wild-type (WT) or mutant (MuT) reporters for COL18A1, THBS2, PTCH1 and PHD3 when co-transfected with the miR-9 mimic. Data are shown as the mean ± s.d. (N.S, no significance; **P < 0.01 and ***P < 0.001; n = 3 independent experiments). d and e Expression of potential targets regulated by miR-9 in A172 miR-9 mimic/NC and U251 miR-9 inhibitor/NC cells was determined by qRT-PCR (d) and western blot analysis (e). Error bars represent the s.d. (*P < 0.05, **P < 0.01 and ***P < 0.001; n = 3 independent experiments)

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