Fig. 3
Mutant p53 negatively regulated miR-223-3p expression. a The p53 sequencing analysis found LSCC cells (NCI-H520, SK-MES-1) bearing missense (215C > G) and nonsense (892G > T) mutations. b ChIP analysis revealed direct binding of mutant p53 to the miR-223 promoter. c Downregulation of p53 in SK-MES-1 and NCI-H520 cells increased miR-223-3p expression but not in NCI-H2170 with wild type p53. d qRT-PCR results showing that miR-223-3p was significantly down-regulated in the LSCC tissues with mutant p53 compared with the LSCC tissues with wild type p53. e Mutant p53215C > G overexpression significantly decreased miR-223-3p expression. **P < 0.01; *P < 0.05; NS: No statistical significance