Fig. 1

Either TSA or PXD101 induces acetylation of histones and inhibits proliferation, migration and invasion of HNSCC cells. a The effects of TSA and PXD101 on acetylation of histones H3 and H4 determined by Western blotting within 4 h after drug treatment. b The effects of TSA and PXD101 on regulation of HDAC activity measured by Abcam’s HDAC Activity Assay Kit (Fluorometric). c The effects of TSA and PXD101 on HNSCC cell proliferation measured by MTS assays within 72 h after drug treatment. d, e The effects of TSA and PXD101 on HNSCC cell migration measured by wound closure assays. Representative images were shown in (d) and quantitative data from three independent experiments were shown in (e). f The effects of TSA and PXD101 on HNSCC cell invasion measured by Borden chambers pre-coated with Matrigel. In this study, 5 μM TSA and PXD101 were used to treat HN12 and HN31 cells. *p < 0.05; **p < 0.01