Fig. 2

TSA downregulates the phosphorylation levels of RTKs in HNSCC cells. a The effect of TSA on global tyrosine phosphorylation determined by Western blotting with anti-phospho-tyrosine PY20 antibody. b, c The effect of TSA on the tyrosine phosphorylation levels of EGFR family members determined by Human Phospho-RTK array. Representative images were shown in (b) and quantitative data from three independent experiments were shown in (c). In this study, 5 μM TSA was used to treat HN12 and HN31 cells. **p < 0.01