Fig. 7
Analysis of gene expression profiles in IL-17A-treated SU-DHL-2 cells. a Heat map of all DEGs after IL-17A treatment. b Heat map of the top 25 most significant up- and down-regulated DEGs ranked by fold change. c The most significant GO biological process terms of upregulated DEGs. d and e The most significant pathways of down- or up-regulated DEGs based on the KEGG database. The co-expression networks of all DEGs (f) and part of DEGs (g) were analyzed using the STRING database and Cytoscape 3.6.0 software. Node size represents the degree of each gene, and the green or red nodes represent down- or up-regulated genes, respectively. Edge size represents the combined score. h Graph of the relative mRNA levels of eight upregulated DEGs in the PI3K/Akt in SU-DHL-2 cells without or with IL-17A (200 pg/ml) co-cultured for 72 h. i Western blot images showing Akt, p-Akt, cyclin D2, and P27 in SU-DHL-2 cells without or with IL-17A (200 pg/ml) co-cultured for 72 h. Error bars represent SD. Significance was determined using two-tailed independent-sample Student’s t/t′ test. (*P < 0.05; **P < 0.01, compared with the SU-DHL-2 group)