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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: EGFR signaling confers resistance to BET inhibition in hepatocellular carcinoma through stabilizing oncogenic MYC

Fig. 1

BET inhibitor inhibits tumor growth more potently than sorafenib in MYC- positive HCC cells. a Cell viability curves are shown for varying doses of JQ1 or sorafenib in BEL-7402 and 97-L cells. Cell viability was determined at 48 h after treatment using Cell Titer-Glo. Values were independently normalized to the untreated cells. b Western blot analysis of BEL-7402 and 97-L cell lysates after treatment with JQ1 or sorafenib for 48 h. The antibodies used are indicated. GAPDH was used as a loading control. c BEL-7402 and 97-L cells were treated with either JQ1 or sorafenib for 48 h. Apoptosis was assessed by Annexin V / PI double staining. Quantification of apoptotic cells was determined based on Annexin V positive cells. ECAR (d) and Glucose uptake values (e) was measured after JQ1 or sorafenib treatment for 24 h by the Seahorse XF Glycolysis Stress Test. BEL-7402 (f) and 97-L cells (g) (5 × 106 each) were injected into the flanks of CB17/SCID mice. Mice were treated with vehicle, JQ1 or sorafenib at 50 mg/kg every 2 days. Tumor volumes were monitored during the treatments. Tumor volumes were assessed using caliper measurements (π/6 × length ×width2); n = 5 for each group. Data are presented as mean ± s.d. *p < 0.05, **p < 0.01

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