Fig. 2
H2S downregulated IDO1 expression by blocking NF-κB and STAT3 pathways. a, b The H2S donor downregulated IDO1 expression in MCF-7 and SGC-7901 cells. MCF-7 and SGC-7901 cells were treated with different concentrations (0–400 μM) of NaHS for 24 h, protein and mRNA expression of IDO1 were detected by western blot and qPCR, respectively. Statistical significance was determined by one-way ANOVA followed by Dunnett’s test. *p < 0.05, bars show the group mean ± SEM. c-f IDO1 expression was regulated by NF-κB and STAT3 pathways in MCF-7 and SGC-7901 cells. Total proteins were extracted from MCF-7 and SGC-7901 cells after being treated with AG490 (0–100 μM) for 24 h, IL-6 (0–50 ng/mL) for 48 h, CAPE (0–25 μM) for 48 h or LPS (0–500 ng/mL) for 24 h and analyzed by western blot. g The H2S donor downregulated IDO1 expression by blocking NF-κB and STAT3 pathways in MCF-7 cells. Total proteins were extracted from MCF-7 cells that were treated with different concentrations of GYY4137 for 24 h and were analyzed by western blot. h H2S deficiency increased the phosphorylation levels of NF-κB and STAT3 pathways in Cse−/− mice. Total proteins were extracted from heart and liver tissues of wt mice and Cse−/− mice, and proteins expression were determined by western blot