Fig. 1

DNA vaccine delivered by EP induces immune responses against neoantigens of B16 tumor model. Six mice per group were vaccinated with three biweekly injections of the B1 or the B2 vaccine (V), which encode neoantigens from the B16 cells in the form of 27 mer peptide as reported in Additional 1: Table S2. (a) Scheme of B1 and B2 vaccine vectors, which encode for ten and two neoantigens, respectively, numbers correspond to neoantigens listed in Additional file 1: Table S2. (b) One week after last immunization, T cell immune responses were analyzed by FC in the peripheral blood for the expression of IFN-γ and TNF-α by FC, gating strategy is shown in Additional file 2: Figure S1, **p < 0.001 Mann-Whitney test. (c) On day 59 six mice per group were challenged with B16 cells (B16) and tumor growth followed over time as described in M&M, bars represent SD