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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Poly-specific neoantigen-targeted cancer vaccines delay patient derived tumor growth

Fig. 3

High affinity MC38 neoantigens are immunogenic. Mice were vaccinated as reported in the scheme with the M2 vaccine, which encodes for the high affinity neoantigens listed in Table 2. (a) Scheme of M2 vaccine. (b) One week after the last vaccination four mice were sacrificed and M2-specific immune responses were analyzed in the splenocytes, value refers to % of CD8+IFN-γ+ gated on CD3+ T cells and measured by FC with the neoantigens peptides listed in Table 2. (c) IFN-γ ELISPOT analysis with splenocytes restimulated with increasing concentration of Reps1 neoantigen and cognate WT peptide the graph on the left shows the number of IFN-γ producing cells after in vitro stimulation of 4 × 105 splenocytes with increasing concentration of peptide; image on the right shows quadruplicate results at 10− 4 μM peptide concentration

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