Fig. 6

Lung tumors from patients with BM showed high LPCAT1 expression and PI3K/AKT/MYC gene signatures. a Representative IHC staining and quantitative analysis showing the expression of LPCAT1 in lung tumors from NSCLC patients with or without BM, and in normal lung tissues. b Heatmap of 566 differentially-expressed genes identified by RNA Sequencing with log2FoldChange more than 2 and P < 0.05 in primary lung tumor tissues from NSCLC patients with or without BM. c Volcano plot revealing the top 60 differentially-expressed genes. d The FPKM value of LPCAT1 expression from RNA-Seq data of BM- and BM+ lung tumor patients. e PCR analysis of LPCAT1 expression in the serum of BM- and BM+ NSCLC patients (n = 10). f Enriched KEGG pathway analysis of differentially expressed genes by DAVID. *P < 0.05, ^#P < 0.001, as determined by the t-test